Ca+ Fura-2 Measurement

HELA cells stained with the Calcium indicator Fura-2.

HELA cells stained with the Calcium indicator Fura-2. Two images of fluorescence at different excitation wavelengths (340nm & 380nm, intensity color coded) were recorded and subsequently the ratio was calculated to achieve a Calcium concentration distribution independent of indicator concentration. Finally the color coded Calcium distribution image was overlaid on a standard microscope image of the cells.
Courtesy of:

T.I.L.L. Photonics GmbH, Gräfelfing, Germany, www.till-photonics.de

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