Imaging Neurons

Images captured using the sensicam qe high performance digital CCD camera.

Hippocampus showing glia (red) and neurons (green)

Darkfield image of an LHRH neuron that took the shape of a question mark.

Neurons that express the mRNA for oxytocin (blue grains represent RNA clusters) were stimulated by suckling and then expressed the immediate early gene Fos (brown nuclei).

Darkfield image of the median eminence showing the site where the LHRH neurons terminate (dense yellow orange structures). Blood vessels in the median eminence drain into the anterior pituitary where LH and FSH are reeased by LHRH.

LHRH neurons (red fluorescent) and in green, nuclei from neurons that are stimulated by a glutamate agonist.

The organum vasculosum of the lamina terminalis is another site where LHRH terminals abound. This darkfield image shows the convergence of LHRH axons in the OVLT.

Brightfield image of neurons that contain norepinephrine (brown) located in the ventrolateral medulla and were stimulated by hypoxia (the black nuclei indicate those cells that were stimulated and expressed an immediate early gene product after the stimulus.

Reproductive neuroendocrine neurons, that make luteinizing hormone releasing hormone, LHRH, (darkfield image; axons are yellow; cell bodies are red outlined in yellow)

 

Courtesy of:

University of Maryland, Gloria E. Hoffman, PH.D., Department of Anatomy and Neurobiology.

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